OcuTox performs drug interaction studies with ocular cell lines and organotypic cultures with RPE-choroid complex with and without retina, cornea or ciliary body in perfusion chambers
Primary RPE cell culture from human
Study endpoints are
Cell viability
Phagocytosis with Image Stream or EM
RPE Hyperthrophy
Drug pigment interaction assessment
Melanin binding
Lysosomal pH
Lysosomal enzyme activity
Protein synthesis
Cell polarity
Protein quantification and detection by immuno cytochemistry
Phospholipidosis
Cellular junctions
Fenestrations
Extracellular matrices
Apoptosis
Morphology
Others